Generator

Part:BBa_K581008:Design

Designed by: Deng Linna   Group: iGEM11_Peking_S   (2011-09-26)


RBS+ccdB+Termintor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 233


Design Notes

Use a relatively weak RBS so the coding sequence would be replicable in non-ccdB resistant strains under the putative promoter in VF region.


Source

Escherichia coli.

References